TY - JOUR
KW - Adolescent
KW - Adult
KW - Age Distribution
KW - Agglutination Tests
KW - Antigens, Protozoan
KW - Child
KW - Child, Preschool
KW - Disinfectants
KW - Enzyme-Linked Immunosorbent Assay
KW - Female
KW - Formaldehyde
KW - Humans
KW - India
KW - Leishmaniasis, Visceral
KW - Male
KW - Middle Aged
KW - Rural Health
KW - Sensitivity and Specificity
KW - Seroepidemiologic Studies
KW - Serologic Tests
KW - Sex Distribution
KW - water
AU - Kumar R
AU - Pai K
AU - Kumar P
AU - Pandey H P
AU - Sundar S
AB - <p><b>OBJECTIVE: </b>To evaluate five kala-azar serological tests for field use.</p><p><b>METHOD: </b>Serological survey in Pandit Ka Purva village in Varanasi district, India, using Sia water test, aldehyde test, direct agglutination test (DAT), micro-enzyme-linked immunosorbent assay (ELISA) and dot-ELISA.</p><p><b>RESULTS: </b>The total population of the village was 518, 67 of whom showed typical clinical and parasitological features of kala-azar, including seven who died. The age distribution of kala-azar cases showed significant differences, being highest among the 45-54-year age group. The disease was more prevalent among males. Serum samples were collected from 498 persons (96% of total population) including 67 kala-azar cases and 40 disease controls (malaria, TB, leprosy, typhoid). Ten 10 serum samples from healthy controls living in endemic area were also collected. The test sensitivities were: Sia water test, 85.0%; aldehyde test, 62.7%; DAT, 94.0%; micro-ELISA, 91.0% and dot-ELISA, 97.0%. The test specificities were: Sia water test 92.5%, aldehyde test, 93.2%, DAT, 96.7; micro-ELISA, 97.6% and dot-ELISA, 98.4%.</p><p><b>CONCLUSION: </b>The dot-ELISA is highly sensitive and specific, cheap, and easy to interpret with the naked eye, making it a powerful screening test for the surveillance and diagnosis of Indian kala-azar at field level.</p>
BT - Tropical medicine & international health : TM & IH
C1 - http://www.ncbi.nlm.nih.gov/pubmed/16398754?dopt=Abstract
DA - 2006 Jan
DO - 10.1111/j.1365-3156.2005.01538.x
IS - 1
J2 - Trop. Med. Int. Health
LA - eng
N2 - <p><b>OBJECTIVE: </b>To evaluate five kala-azar serological tests for field use.</p><p><b>METHOD: </b>Serological survey in Pandit Ka Purva village in Varanasi district, India, using Sia water test, aldehyde test, direct agglutination test (DAT), micro-enzyme-linked immunosorbent assay (ELISA) and dot-ELISA.</p><p><b>RESULTS: </b>The total population of the village was 518, 67 of whom showed typical clinical and parasitological features of kala-azar, including seven who died. The age distribution of kala-azar cases showed significant differences, being highest among the 45-54-year age group. The disease was more prevalent among males. Serum samples were collected from 498 persons (96% of total population) including 67 kala-azar cases and 40 disease controls (malaria, TB, leprosy, typhoid). Ten 10 serum samples from healthy controls living in endemic area were also collected. The test sensitivities were: Sia water test, 85.0%; aldehyde test, 62.7%; DAT, 94.0%; micro-ELISA, 91.0% and dot-ELISA, 97.0%. The test specificities were: Sia water test 92.5%, aldehyde test, 93.2%, DAT, 96.7; micro-ELISA, 97.6% and dot-ELISA, 98.4%.</p><p><b>CONCLUSION: </b>The dot-ELISA is highly sensitive and specific, cheap, and easy to interpret with the naked eye, making it a powerful screening test for the surveillance and diagnosis of Indian kala-azar at field level.</p>
PY - 2006
SP - 41
EP - 8
T2 - Tropical medicine & international health : TM & IH
TI - Sero-epidemiological study of kala-azar in a village of Varanasi district, India.
VL - 11
SN - 1360-2276
ER -