TY - JOUR
KW - onchocerciasis
KW - Ov16 ELISA serological assessment
KW - O-150 PCR entomological assessment
KW - interruption of transmission
KW - Elimination
KW - stop-mass drug administration
AU - Ityonzughul C
AU - Sallau A
AU - Miri E
AU - Emukah E
AU - Kahansim B
AU - Adelamo S
AU - Chiedo G
AU - Ifeanyichukwu S
AU - Coalson JE
AU - Rakers L
AU - Griswold E
AU - Makata C
AU - Oyediran F
AU - Osuji S
AU - Offor S
AU - Obikwelu E
AU - Otiji I
AU - Richards FO
AU - Noland GS
AB - <jats:p>Onchocerciasis causes severe morbidity in sub-Saharan Africa. Abia, Anambra, Enugu, and Imo states of Nigeria were historically classified meso- or hyperendemic and eligible for ivermectin mass drug administration (MDA). After ≥25 years of annual and biannual MDA, serological and entomological assessments were conducted to determine if Onchocerca volvulus transmission was interrupted. Dried blood spots collected in October 2020 from ≥3167 children 5–9 years old in each state were screened for O. volvulus-specific Ov16 antibody by enzyme-linked immunosorbent assay. Additionally, 52,187 Simulium damnosum heads (≥8845 per state) collected over 12 months between 2021 and 2022 were tested by pooled polymerase chain reaction (PCR) for O-150 DNA. Among seven seropositive children, four were found for follow-up skin snip PCR to confirm active infection. Three were negative and the fourth was excluded as he was visiting from an endemic state. The final seroprevalence estimates of each state had 95% upper confidence limits (UCL) &lt; 0.1%. All fly pools were negative by O-150 PCR, giving a 95% UCL infective fly prevalence &lt; 0.05% in each state. Each state therefore met the World Health Organization epidemiological and entomological criteria for stopping MDA effective January 2023. With 18.9 million residents eligible for MDA, this marked the largest global onchocerciasis stop-treatment decision to date.</jats:p>
BT - Pathogens
DO - 10.3390/pathogens13080671
IS - 8
LA - ENG
M3 - Article
N2 - <jats:p>Onchocerciasis causes severe morbidity in sub-Saharan Africa. Abia, Anambra, Enugu, and Imo states of Nigeria were historically classified meso- or hyperendemic and eligible for ivermectin mass drug administration (MDA). After ≥25 years of annual and biannual MDA, serological and entomological assessments were conducted to determine if Onchocerca volvulus transmission was interrupted. Dried blood spots collected in October 2020 from ≥3167 children 5–9 years old in each state were screened for O. volvulus-specific Ov16 antibody by enzyme-linked immunosorbent assay. Additionally, 52,187 Simulium damnosum heads (≥8845 per state) collected over 12 months between 2021 and 2022 were tested by pooled polymerase chain reaction (PCR) for O-150 DNA. Among seven seropositive children, four were found for follow-up skin snip PCR to confirm active infection. Three were negative and the fourth was excluded as he was visiting from an endemic state. The final seroprevalence estimates of each state had 95% upper confidence limits (UCL) &lt; 0.1%. All fly pools were negative by O-150 PCR, giving a 95% UCL infective fly prevalence &lt; 0.05% in each state. Each state therefore met the World Health Organization epidemiological and entomological criteria for stopping MDA effective January 2023. With 18.9 million residents eligible for MDA, this marked the largest global onchocerciasis stop-treatment decision to date.</jats:p>
PB - MDPI AG
PY - 2024
SP - 1
EP - 12
T2 - Pathogens
TI - The Interruption of Transmission of Onchocerciasis in Abia, Anambra, Enugu, and Imo States, Nigeria: The Largest Global Onchocerciasis Stop-Treatment Decision to Date
UR - https://www.mdpi.com/2076-0817/13/8/671/pdf?version=1723096333
VL - 13
SN - 2076-0817
ER -