02687nas a2200385   4500000000100000008004100001260003500042653004500077653002200122653002800144653002700172653001900199653001400218653001900232653001000251653001500261653001200276100001400288700001500302700001300317700002000330700001500350700001200365700001400377700001400391700001400405700001500419700001300434245008300447856006900530300001200599490000700611520165800618022002502276       2023                            d  bAfrican Journals Online (AJOL)10aIndustrial and Manufacturing Engineering10aMetals and Alloys10aStrategy and Management10aMechanical Engineering10aonchocerciasis10adiagnosis10aIgG4 OV-16 RDT10aELISA10aOgun State10aNigeria1 aSurakat O1 aSam-Wobo S1 aGolden A1 aDe Los Santos T1 aAdeleke MA1 aFaulx D1 aAdemolu K1 aBankole S1 aKalnoky M1 aStevens, E1 aYokobe L00aField test and sensitivity of Onchocerciasis rapid test in Ogun State, Nigeria  uhttps://www.ajol.info/index.php/rejhs/article/view/249942/236304  a213-2230 v113 a<p><strong>Objective</strong>: This study, conducted between March 2015 and July 2016, investigated the sensitivity and specificity of the OV-16 RDT and Dried Blood Spot (DBS) Enzyme-Linked Immunosorbent Assay (ELISA) for skin snip microfilaria (MF) detection in Ogun State.</p>

<p><strong>Methods:</strong> Five hundred and eighty-eight (588) members of 16 first-line communities in 8 endemic Local Government Areas (LGAs) provided fingerstick whole blood specimens examined for IgG4 antibodies against the O. volvulus antigen OV-16, using DBS OV-16 ELISA and OV-16 RDT. In consort with these specimens, 162 study participants consented to be evaluated by skin snip.</p>

<p><strong>Results:</strong> Results showed a seroprevalence of 106(18.0%) using ELISA and 102(17.3%) by RDT, with 92.2% agreement. The OV-16 RDT demonstrated a positive and negative agreement of 76.4 (95% CI 67.2 to 84.1%) and 95.6% (95% CI: 93.4 to 97.3), respectively, to the OV-16 ELISA results from the concordant dataset. Against the 64 positive skin snips microscopy results, sensitivity of the RDT was 79.7% (95% CI 73.43% to 85.27) and the ELISA was 70.3% (95% CI 64.72% to 75.37%).</p>

<p><strong>Conclusion</strong>: The results of this study provided information on the performance of the OV-16 RDT. This information will contribute data for comparison to studies in other areas where treatment is ongoing. In conclusion, the commercial launch of the OV-16 RDT has made available a point-of-care diagnostic tool that could serve to help local and country onchocerciasis elimination programs to measure changes and progress in onchocerciasis intervention.</p>
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