03564nas a2200181   4500000000100000008004100001260003300042100001100075700001300086700001400099700001300113700001400126700001400140700001600154245009500170856006000265520305700325       2021                            d  bResearch Square Platform LLC1 aDabo A1 aDiallo M1 aAgniwo PK1 aDanté S1 aTraoré A1 aDiawara S1 aDoucouré B00aMass Drug Distribution Strategy Efficacy for Schistosomiasis Control in Mali (West Africa)  uhttps://www.researchsquare.com/article/rs-480049/v1.pdf3 a<jats:title>Abstract</jats:title>
        <jats:p><jats:bold><jats:italic>Background - </jats:italic></jats:bold>Schistosomiasis is a water-based parasitic disease caused by blood flukes of the genus <jats:italic>Schistosoma. </jats:italic>Mass Drug Distribution (MDD) with Praziquantel is periodically recommended in schistosomiasis-endemic countries to prevent morbidity. In Mali, schistosomiasis still remains endemic, especially in Senegal and Niger rivers basin, although the strategy has been adopted since 2005. Our study aimed to assess the impact of annual school-based MDD. <jats:bold><jats:italic>Materials and methods – </jats:italic></jats:bold>The study was conducted at twelve sentinel sites across Kayes and Koulikoro regions. A cross-sectional study design was performed in April 2018 after four-or five-years post treatment with praziquantel (PZQ) depending on the statute of sites. At baseline (2014-2015) and for the control year (2018), 734 (386 boys and 347 girls) and 1708 (844 boys and 864 girls) school children of 7-14 years of age, were successfully examined respectively. Infections with <jats:italic>Schistosoma haematobium</jats:italic> and <jats:italic>S. mansoni</jats:italic> were diagnosed with the urine filtration and the Kato-Katz method respectively. <jats:bold><jats:italic>Results </jats:italic></jats:bold>– From eight schools treated in 2014, the four annual rounds of MDD with PZQ were associated with a significant decrease in <jats:italic>S. haematobium</jats:italic> prevalence in five sites (<jats:italic>p&lt;0,001)</jats:italic> and a significant increase in one site (<jats:italic>p&lt;0,001</jats:italic>)<jats:italic>. </jats:italic>Of them, the prevalence of high-intensity (≥50 eggs/10mL of urine) significantly decreased in four previous sites but not in Diakalele where it increased ((<jats:italic>p&lt;0.001). </jats:italic>In all the four sites treated in 2015, <jats:italic>S. haematobium</jats:italic> prevalence increased significantly in Kokoun and Samaya (<jats:italic>P&lt;0.05</jats:italic>). The heavy infection increased slightly in Dougourakoro and Samaya (<jats:italic>P&gt;0.05</jats:italic>). The prevalence of <jats:italic>S. mansoni</jats:italic> significantly decreased in three sites (<jats:italic>p&lt;0.001</jats:italic>), increased in three and remains zero in the six others sites. <jats:bold>C<jats:italic>onclusion</jats:italic></jats:bold> – Our findings show that five of the twelve sentinel sites have achieved the criterion of elimination of schistosomiasis due to <jats:italic>Schistosoma haematobium </jats:italic>as a major public health problem (Prevalence of heavy infection &lt;1%); three have achieved the criterion of morbidity control (Prevalence of heavy infection &lt;5%), whereas two sites remain confined below the control criterion. These results call for a strong improvement in the therapeutic coverage associating, education and provision of safe water, sanitation and hygiene to interrupt the transmission cycle of the schistosome.</jats:p>