02212nas a2200361   4500000000100000008004100001260000900042653003800051653003000089653001100119653001500130653001800145653002100163653002300184653001400207653002500221653002000246653003000266100001300296700001100309700001200320700001700332700001600349700001500365700001900380700001300399245012300412856012100535300001100656490000600667520116300673022001401836       2013                            d  c201310aEnzyme-Linked Immunosorbent Assay10aHSP70 Heat-Shock Proteins10aHumans10aLeishmania10aLeishmaniasis10aOligonucleotides10aProtozoan Proteins10aROC Curve10aRecombinant Proteins10aSerologic Tests10aStatistics, Nonparametric1 aSouza AP1 aSoto M1 aCosta J1 aBoaventura V1 aOliveira CI1 aCristal JR1 aBarral-Netto M1 aBarral A00aTowards a more precise serological diagnosis of human tegumentary leishmaniasis using Leishmania recombinant proteins.  uhttp://www.plosone.org/article/fetchObject.action?uri=info%3Adoi%2F10.1371%2Fjournal.pone.0066110&representation=PDF  ae661100 v83 a<p><b>BACKGROUND: </b>Exposure to Leishmania induces a humoral immune response that can be used as a marker of parasite exposure.</p><p><b>METHODOLOGY/PRINCIPAL FINDINGS: </b>Herein, ELISA was used to screen sera from patients with Tegumentary Leishmaniasis (TL) against different L. infantum-chagasi-derived recombinant proteins (rHSP70, rH2A, rH2B, rH3, rH4 and rKMP11). Among the recombinant proteins, rHSP70 and rH2A showed the best reactivity against human sera obtained from endemic areas of TL. Receiver-Operator Characteristics (ROC) curve analysis was used to identify the effectiveness of these proteins for serodiagnosis of TL. ROC curves confirmed the superior performance of rHSP70 and rH2A, in comparison to the other tested recombinant proteins. Additionally, we evaluated the specificity of the response to rHSP70 and rH2A by testing sera obtained from patients with Chagas' disease, Tuberculosis, Leprosy or Systemic Lupus Erythematosus. In this case, rHSP70 displayed an increased ability to discriminate diseases, in comparison to SLA.</p><p><b>CONCLUSION: </b>Our results raise possibility of using rHSP70 for the serodiagnosis of TL.</p>  a1932-6203