01477nas a2200289   4500000000100000008004100001260001300042653001500055653002000070653002800090653003800118653001300156653001100169653002800180653001800208653003300226653002800259100001200287700001400299700001400313700001500327245011300342300001000455490000700465520070100472022001401173       1980                            d  c1980 Mar10aAntibodies10aCross Reactions10aDiagnosis, Differential10aEnzyme-Linked Immunosorbent Assay10aEpitopes10aHumans10aImmunoenzyme Techniques10aLeishmaniasis10aLeishmaniasis, Mucocutaneous10aLeishmaniasis, Visceral1 aRoffi J1 aDedet J P1 aDesjeux P1 aGarré M T00aDetection of circulating antibodies in cutaneous leishmaniasis by enzyme-linked immunosorbent assay (ELISA).  a183-90 v293 a<p>An immunoenzymatic diagnostic technique applicable to cutaneous leishmaniasis is described. The antigen used (Leishmania tropica major) was equally useful in diagnosing visceral and mucocutaneous forms of the disease. The criteria for positivity were defined by using groups of negative controls, and the specificity of the reaction was evaluated by using sera from patients with various diseases. Among these, sera from patients with lepromatous leprosy, tuberculosis, or African trypanosomiasis strongly cross-reacted with leishmania antigen. Examining serial dilutions of the sera facilitated the interpretation of the results and eliminated a significant percentage of false positives.</p>  a0002-9637