02653nas a2200433   4500000000100000008004100001260001200042653001900054653003800073653003900111653003300150653001600183653003400199100001800233700001300251700001100264700001300275700001500288700001400303700001300317700002000330700001500350700001300365700001500378700001300393700001500406700001200421700001200433700001500445700001200460700001600472700001400488245017100502856006700673300000900740490000700749520144900756022001402205       2024                            d  bMDPI AG10aonchocerciasis10aOv16 ELISA serological assessment10aO-150 PCR entomological assessment10ainterruption of transmission10aElimination10astop-mass drug administration1 aItyonzughul C1 aSallau A1 aMiri E1 aEmukah E1 aKahansim B1 aAdelamo S1 aChiedo G1 aIfeanyichukwu S1 aCoalson JE1 aRakers L1 aGriswold E1 aMakata C1 aOyediran F1 aOsuji S1 aOffor S1 aObikwelu E1 aOtiji I1 aRichards FO1 aNoland GS00aThe Interruption of Transmission of Onchocerciasis in Abia, Anambra, Enugu, and Imo States, Nigeria: The Largest Global Onchocerciasis Stop-Treatment Decision to Date  uhttps://www.mdpi.com/2076-0817/13/8/671/pdf?version=1723096333  a1-120 v133 a<jats:p>Onchocerciasis causes severe morbidity in sub-Saharan Africa. Abia, Anambra, Enugu, and Imo states of Nigeria were historically classified meso- or hyperendemic and eligible for ivermectin mass drug administration (MDA). After ≥25 years of annual and biannual MDA, serological and entomological assessments were conducted to determine if Onchocerca volvulus transmission was interrupted. Dried blood spots collected in October 2020 from ≥3167 children 5–9 years old in each state were screened for O. volvulus-specific Ov16 antibody by enzyme-linked immunosorbent assay. Additionally, 52,187 Simulium damnosum heads (≥8845 per state) collected over 12 months between 2021 and 2022 were tested by pooled polymerase chain reaction (PCR) for O-150 DNA. Among seven seropositive children, four were found for follow-up skin snip PCR to confirm active infection. Three were negative and the fourth was excluded as he was visiting from an endemic state. The final seroprevalence estimates of each state had 95% upper confidence limits (UCL) &lt; 0.1%. All fly pools were negative by O-150 PCR, giving a 95% UCL infective fly prevalence &lt; 0.05% in each state. Each state therefore met the World Health Organization epidemiological and entomological criteria for stopping MDA effective January 2023. With 18.9 million residents eligible for MDA, this marked the largest global onchocerciasis stop-treatment decision to date.</jats:p>  a2076-0817