01899nas a2200253   4500000000100000008004100001260001200042653001900054653001400073653002900087653001800116653002700134653001700161100001500178700002100193700001300214700001300227245009300240856007100333300000900404490000700413520121100420022001401631       2024                            d  c08/202410aChagas disease10adiagnosis10aIsothermal amplification10aPoint-of-care10aRapid diagnostic tests10aSurveillance1 aSchijman A1 aAlonso-Padilla J1 aBritto C1 aBernal C00aRetrospect, advances and challenges in Chagas disease diagnosis: a comprehensive review.  uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11246061/pdf/main.pdf  a1-140 v363 a<p>Chagas disease, caused by , affects millions worldwide. The 2030 WHO roadmap aims to eliminate it as a public health concern, emphasising the need for timely diagnosis to enhance treatment access. Current diagnostic algorithms, which rely on multiple tests, have prolonged turnaround times. This proves particularly problematic in resource-limited settings. Addressing this issue necessitates the validation and adoption of innovative tools. We explore recent developments in Chagas disease diagnosis, reviewing historical context and advancements. Despite progress, challenges persist. This article contributes to the understanding of current and future directions in this neglected healthcare area. Parasitological methods are simple but exhibit low sensitivity and require supplementary tests. Molecular methods, with automation potential, allow quantification and higher throughput. Serological tools show good performance but struggle with parasite antigenic diversity. Prioritising point-of-care tests is crucial for widespread accessibility and could offer a strategy to control disease impact. Ultimately, balancing achievements and ongoing obstacles is essential for comprehensive progress.</p>  a2667-193X