@article{96529,
  keywords = {General Biochemistry, Genetics and Molecular Biology},
  author = {You H and Gordon CA and MacGregor SR and Cai P and McManus DP},
  title = {Potential of the CRISPR‐Cas system for improved parasite diagnosis},
  abstract = {<p>CRISPR-Cas technology accelerates development of fast, accurate, and portable diagnostic tools, typified by recent applications in COVID-19 diagnosis. Parasitic helminths cause devastating diseases afflicting 1.5 billion people globally, representing a significant public health and economic burden, especially in developing countries. Currently available diagnostic tests for worm infection are neither sufficiently sensitive nor field-friendly for use in low-endemic or resource-poor settings, leading to underestimation of true prevalence rates. Mass drug administration programs are unsustainable long-term, and diagnostic tools – required to be rapid, specific, sensitive, cost-effective, and user-friendly without specialized equipment and expertise – are urgently needed for rapid mapping of helminthic diseases and monitoring control programs. We describe the key features of the CRISPR-Cas12/13 system and emphasise its potential for the development of effective tools for the diagnosis of parasitic and other neglected tropical diseases (NTDs), a key recommendation of the NTDs 2021–2030 roadmap released by the World Health Organization.</p>
},
  year = {2022},
  journal = {BioEssays},
  pages = {2100286},
  publisher = {Wiley},
  issn = {0265-9247, 1521-1878},
  url = {https://onlinelibrary.wiley.com/doi/epdf/10.1002/bies.202100286},
  doi = {10.1002/bies.202100286},
  language = {eng},
}